■ 基本信息
啟動子: | T7 |
復(fù)制子: | M13 |
質(zhì)粒分類: | 大腸桿菌載體；蛋白表達質(zhì)粒 |
質(zhì)粒大小: | 7477bp |
質(zhì)粒標簽: | Intein |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達宿主: | 大腸桿菌 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據(jù)序列設(shè)計 |
備注: | 表達質(zhì)粒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡介
The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS upstream of the Sce VMA intein. The NdeI site in the MCS has the initiator sequence ATG that enables translation of the ORF. The chitin-binding domain(CBD) downstream of the intein results in a target protein-intein with C-terminal chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK335pTYB1大腸表達質(zhì)粒.txt
