■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal,hGH poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，蛋白過表達(dá)載體 |
質(zhì)粒大小: | 6310bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測(cè)序引物: | hGH-PA-R:CCAGCTTGGTTCCCAATAGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418 |
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
P3xFlag-CMV-14 expression vector is a 6.3 KB carrier derived from the pCMV5 vector, which is used for transient expression in mammalian cells or the stable expression of C terminal with 3xFlag tag fusion protein. Three adjacent Flag epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) were encoded by the downstream fragment of the vector. This makes it possible to detect protein expression using M2 Flag antibody.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK379p3×FLAG-CMV-14哺乳表達(dá)質(zhì)粒.txt
