■ 基本信息
啟動(dòng)子: | CAG |
復(fù)制子: | pUC |
終止子: | β-globin poly(A) signal |
質(zhì)粒分類: | 哺乳系列質(zhì)粒；哺乳表達(dá)質(zhì)粒；pCAG系列質(zhì)粒 |
質(zhì)粒大小: | 4801bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，5%CO2 |
表達(dá)宿主: | 293T等哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)需誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | pcaggs-F（GTTCGGCTTCTGGCGTGT） |
3'測(cè)序引物: | pcaggs-R（TATGTCCTTCCGAGTGAGAG） |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
該質(zhì)粒可用于在雞b-肌動(dòng)蛋白，兔b-球蛋白雜合啟動(dòng)子（CAG）的控制下高效表達(dá)基因，和各種哺乳動(dòng)物細(xì)胞中的人CMV-IE增強(qiáng)子; CAG啟動(dòng)子序列由雞b-肌動(dòng)蛋白啟動(dòng)子，第一外顯子和第一內(nèi)含子的一部分（似乎具有強(qiáng)的增強(qiáng)子樣活性），與兔b-珠蛋白片段連接，由第二內(nèi)含子的3'部分 包括正常拼接反應(yīng)所需的分支點(diǎn)）和第三外顯子的5'部分。
This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK399pCAGGS哺乳表達(dá)質(zhì)粒.txt
